RAPIDCLICK TARGETING SERIES

Of covalent thioether bonds between maleimide and cysteine groups Sulfhydryl-based linker chemistry for NE conjugation by the formation 3 This requires chemical modification of the surface of theĭroplets to introduce functional groups, e.g., amine, carboxyl, or Surface after the emulsification process is complete. In the post-emulsification approach, the ligand is conjugated to the To withstand the extreme shear forces imposed by the homogenization. Method may be simplistic to implement, ligands must be robust enough Microfluidization) to form the stabilized NE. 16 Pre-emulsification techniques rely on ligandĬonjugation before the high-pressure, homogenization process (i.e., Two distinct methods, including pre- and post-emulsification approaches. 14, 15 Strategies for NE surface functionalization have been reported using Polymers, dyes, and chelates are of the greatest interest. Probes harboring a variety of ligand types such as peptides, antibodies, Our goal is to adorn NE with ligands bearingĬellular biomarkers as well as moieties for multimodal imaging. 11, 12 Overall, 19F MRI detection is feasible when 10 3–10 5 cells locally accumulate to generate an image hot-spot. The utility of 19F MRI has beenĭemonstrated for imaging stem cells, 2, 8 various immuneĬell subsets, 9, 10 macrophage-associated inflammatoryĭiseases, and regenerative medicine. NE is injected intravenously, and droplets are taken up by phagocyticĬells of the reticuloendothelial system (RES), including macrophagesĪt sites of inflammation. Of emerging cytotherapies used to treat cancer. This approach is particularly useful for following the fate Cell uptakeĬan be achieved by simple coincubation with cells of interest ex vivo, followed by delivery of the labeled cells to the Suspensions of PFC droplets, stabilized using a surfactant. Most often formulated as nanoemulsions (NEs), which are colloidal To their F-dense composition, which affords high MRI sensitivity as Perfluorocarbon (PFC) oils are a common fluorine tracer agent due 3, 4 Coincident 1H MRI provides the anatomical context for image interpretation. MRI due to the relatively high sensitivity, which is comparable to 1H nuclei, and its low background in biological tissues compared Tracking is a relatively new use for MRI that is making inroads towardĪn attractive tracer probe for in vivo “hot-spot” Visualizing soft-tissue anatomical morphology at high-resolution and Imaging (MRI) is a powerful technique for noninvasively Overall, these dataĭemonstrate the potential of PFC nanoemulsions incorporating DBCOĪs a versatile platform for generating functionalized probes. Of the fluorescent PFC by macrophages was demonstrated both in vitro in cultured macrophages and in situ in an acute inflammation mouse model, where fluorescence imagingĪnd 1H/ 19F magnetic resonance imaging (MRI) The feasibility of functionalization the by click reaction. As proof-of-concept,įluorescent dye-azides were conjugated to PFC nanoemulsions, demonstrating The addition of DBCO-lipid to the surfactant in PFC nanoemulsionsĭid not affect nanoemulsion size or nanoemulsion stability. Incorporating dibenzocyclooctyne (DBCO) by enabling postemulsificationįunctionalization via a click reaction with azide-containing ligands. This work, we demonstrate the utility of perfluorocarbon (PFC) nanoemulsions Targeting moieties and payloads for multimodal image generation. That is readily modifiable to accommodate binding of different molecular Your regular office visit copay, coinsurance, or deductible applies.We describe an in vivo imaging probe platform Inquire with Target Clinic to see if you’re eligible for a one-time fill. Refills must be obtained from a Kaiser Permanente pharmacy. For the complete definition of an emergency medical condition, please refer to your Evidence of Coverage or other coverage documents.Įligible Kaiser Permanente members can fill new prescriptions as a result of a visit to a Target Clinic at the co-located CVS Pharmacy at Target. If you believe you have an emergency medical condition, call 911 or go to the nearest hospital.

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RAPIDCLICK TARGETING SERIES

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